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ATCC
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European Collection of Authenticated Cell Cultures
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SV40 transformedTumorigenecity: Isoenzyme: G6PD, BHistopathology: SV40 transformedSubculture: Split ratio: Media change: Reverse transcritase: Production.
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Image Search Results
Journal: Experimental & Molecular Medicine
Article Title: TFAP2C-mediated upregulation of TGFBR1 promotes lung tumorigenesis and epithelial–mesenchymal transition
doi: 10.1038/emm.2016.125
Figure Lengend Snippet: TGFBR1 (transforming growth factor-β receptor type 1) was upregulated by TFAP2C (transcription factor-activating enhancer-binding protein 2C) in non-small-cell lung cancer (NSCLC) cells. ( a ) The effects of TFAP2C knockdown by treatment of TFAP2C small interfering RNA (siRNA) (#1 and #2) on the expression of TGFBR1 mRNA and protein in NCI-H292 and NCI-H838 cells were analyzed by real-time quantitative RT-PCR (qRT-PCR) and western blotting, respectively. Based on these results, TFAP2C siRNA no 1 was used for the subsequent experiments. ( b ) The effects of TFAP2C upregulation on the expression of TGFBR1 mRNA and protein in MRC5 and WI-26 VA4 cells were analyzed by real-time qRT-PCR and western blotting, respectively. * P <0.05 compared with control cells. Error bars represent±s.e.m. ( n =3); Student's t -test.
Article Snippet: The human NSCLC cell lines, NCI-H292 and NCI-H838, and the human normal lung cell lines, MRC5 and
Techniques: Binding Assay, Knockdown, Small Interfering RNA, Expressing, Quantitative RT-PCR, Western Blot, Control